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hace 2 meses
The Centre De Regulació Genòmica (Crg) is seeking a highly skilled Stem Cell Specialist to join our Tissue Engineering Unit. As a key member of our team, you will be responsible for providing patient-derived organoid (PDOs), mouse and human pluripotent 2D (monolayer) and 3D (organoids) stem cell differentiation procedures already established in the Unit. You will also execute CRISPR/Cas9 gene editing services and set up specific 2D/3D differentiation and gene editing procedures upon user request for specific projects.
Key Responsibilities- Provide patient-derived organoid (PDOs), mouse and human pluripotent 2D (monolayer) and 3D (organoids) stem cell differentiation procedures
- Execute CRISPR/Cas9 gene editing services
- Set up specific 2D/3D differentiation and gene editing procedures upon user request
- Work collaboratively with the team to achieve project goals
- At least 3 years' experience in stem cell biology and ES/iPS cell differentiation systems
- At least 1 year experience in CRISPR/CAS9 or related gene editing system
- Experience in working in a Core Facility or Industry/Biotech/R&D Facility
- Strong Tissue Culture experience for human and mouse ES/iPS cells and PDOs
- Strong experience in ES cell differentiation
- Experience in cell lines gene editing
- Experience with immunofluorescence, confocal, and FACS analysis for cellular characterizations
- Master/PhD Degree in biology or a related field
- Fluent in English
- Proficient in Spanish
- Technical and scientific activities contract – estimated 1 year with possibility of extension
- Estimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales