Vac_55_2024_Immerge: Doctoral Candidate Position_European Molecular Biology Laboratory Heidelbe...

hace 4 semanas


Badalona, España Institut Josep Carreras (Ijc) A tiempo completo

.Your missionHOST INSTITUTION: European Molecular Biology Laboratory Heidelberg (EMBL), GermanyHeidelbergDegree awarding institution: EMBL, Heidelberg, GermanySupervisor: Dr. Sinem SakaPlanned secondment(s):UKLFR to receive training on patient sample processing and learn about signaling pathways, m10-m12 (2 months); Alia Therapeutics to learn novel gene editing protocols, m18 (1 month); University of Basel to receive multi-omics analysis, m23-24 (2 months).In the realm of cutting-edge research and academic excellence, the IMMERGE MSCA Doctoral Network (DN) stands as an extraordinary training initiative, embracing a multidisciplinary perspective that spans various domains of knowledge.The program is dedicated to recruit 12 remarkable Doctoral Candidates (DCs) within the fields of Immunology, Epigenetics, Omics and Gene Editing Technologies and Bioinformatics.The objective of the program is to answer several essential questions regarding the Inborn Errors of Immunity (IEI), such as what is the relationship between genetic mutations and the wide clinical phenotypic expressivity and drug response in different individuals?How are the specific effects of these mutations modulated by the genetic background and/or environmental factors? What is the interplay between these mutations and a wealth of cell signaling pathways and transcriptional factors? Can we correct them?Inborn Errors of Immunity (IEI) represent a paradigm for (i) exploring the communication between genetics, epigenetic, and environmental determinants and (ii) testing the potential of gene editing methods.The DCs will be enrolled in top-notch academic and non-academic partners working with experts in immunology, genetics, epigenetics, proteomics, single cell omics, bioinformatics, and gene correction.Participating institutions represent 7 different countries, with 8 members in academia and 2 non-academic, from the biotech sector.In addition, the overall programme has designed a tailored training programme in which 16 Associated Partners bring additional expertise to the MSCA DN.Individual Research ProjectDoctoral Candidate 13: Understanding the impact of IEI on RNA processing and lifecycle with new methods.Rationale and Objectives: For IEIs that do not impede transcription, it is unclear if the produced RNA suffers from aberrations throughout its lifecycle such as mislocalization, delayed splicing, translation, or lowered lifetime.DC13 will study how IEIs affect the downstream processing and lifecycle of mutant RNAs by comparatively studying the impact on the RNA lifecycle dynamics of mutant versus wildtype RNAs.Specific aims for DC13's project are:To develop a highly sensitive in situ proximity detection method that will allow annotating RNAs based on their functional state and hence study their lifecycle stage at single-molecule resolution in multiplexed fashion, under conditions of disease and treatment



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