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The Josep Carreras Leukaemia Research Institute (IJC) is a comprehensive **cancer research center** dedicated to conduct research and drive innovation in the epidemiological, preventive, clinical, translational, and basic aspects of leukaemia and other hematologic malignancies, with the final aim of finding a cure for these diseases. It was created in 2010, and since then has experienced an exponential growth.
The IJC is also part of the network of Excellence Research Centers of Catalonia **(CERCA)** and was accredited as a **Severo Ochoa Center of Excellence** by the Ministry of Science, Innovation, and Universities in 2024. Since 2018, the Institute has also been accredited by the Scientific Foundation of the Spanish Association Against Cancer **(FCAECC)** Additionally, IJC is one of the centers integrated into the Institut de Recerca Germans Trias i Pujol (IGTP) accreditation as Accredited Health Research Institute (IIS) by the Carlos III Health Institute **(ISCIII)**.
The IJC's main headquarters is located within the Can Ruti Biomedical Campus, alongside other leading biomedical institutions, providing direct access to cutting-edge scientific and technological facilities, as well as complementary community services. The IJC has **six locations integrated into reference hospitals**: Hospital Germans Trias i Pujol, Hospital Clínic, Hospital Sant Pau, Hospital Josep Trueta, Hospital del Mar and Hospital San Joan de Déu. This facilitates close collaboration between basic and clinical researchers, fostering translational research that integrates basic science with clinical practice in clinical settings.
The Josep Carreras Leukemia Research Institute is looking for a **Predoctoral Researcher** for the **Epigenetics and immune disease** group led by Dr. Esteban Ballestar. The contract will be linked to project PID2024-156688NB-I00/MICIU/AEI/10.13039/501100011033/ FSE+.
**RESEARCH DESCRIPTION**
Our group investigates the epigenetic mechanisms that shape the functional diversity of immune cells in health and disease, process that are highly relevant to hematological malignancies. Building on extensive experience in DNA methylation, single-cell omics and immunology, our research now focuses on unraveling how transcriptional and epigenomic programs are established in pathological niches with profoundly altered immune environments.
The EPiMAP project (“Revealing Epigenetic Processes Regulating Myeloid Adaptation and Reprogramming in Pathological Niches”) aims to dissect how extracellular cues, cell-cell communication and signaling pathways determine myeloid cell identity and function within two clinically relevant contexts:
- the hypoxic tumor microenvironment (TME), characterized by immunosuppressive macrophages and myeloid-derived suppressor cells, and
- the inflamed tissues in autoimmune diseases, where pro-inflammatory myeloid cells drive tissue destruction.
Through the integration of single-cell RNA-seq/ATAC-seq, spatial transcriptomics, DNA methylation profiling (WGBS/EPIC arrays), CUT&Tag, organoid and co-culture models, and CRISPR perturbation, we will define regulatory networks and identify epigenetic and transcriptional determinants that confer immunosuppressive or immunogenic programs in human myeloid cells. These studies will help reveal actionable pathways for therapeutic modulation of myeloid responses in cancer and inflammatory disease
**MAIN RESPONSABILITIES**
- **Participate in the characterization of immune cells** from tumor microenvironment samples and autoimmune tissues using single-cell and spatial omics technologies.
- **Contribute to epigenomic analyses**, including DNA methylation (WGBS/EPIC), CUT&Tag, RNA-seq and ATAC-seq, and assist in dataset processing with support from senior team members.
- **Perform in vitro differentiation experiments** with monocytes and CD34+ stem cells to generate macrophages and dendritic cells, and help test the effects of selected signaling pathways, transcription factors and epigenetic modulators.
- **Assist with co-culture and organoid models** to study myeloid cell adaptation in tumor and inflammatory environments.
- **Perform bioinformatic analysis**, integration of multi-omics data and organization of project datasets.
- **Prepare figures, reports and presentations**, and contribute to internal meetings, collaborations with clinicians, and dissemination of results.
**WHAT WE NEED**
- Master’s degree in Biomedical Sciences or a related discipline.
- Strong interest and motivation to pursue a research career in the biomedical field.
- Organized, proactive, self-motivated, and enthusiastic.
- Excellent academic performance.
- Proven ability to work both independently and as part of a team.
- Proactive attitude, creativity, and a collaborative mindset.
- Capacity to manage multiple tasks and adapt to varying workloads.
- Strong interpersonal and communication skills.
- Proficient in writing and speaking in English.
**WHAT WE OFFER**
- A FPI PhD
