Research Support Technician PR27/01/2026
hace 5 días
Organisation/Company Autonomous University of Madrid (Universidad Autónoma de Madrid) Department Teaching and Research Staff (Personal Docente e Investigador) Research Field Biological sciences Researcher Profile First Stage Researcher (R1) Positions Master Positions Application Deadline 2 Feb 2026 - 00:00 (Europe/Madrid) Country Spain Type of Contract Permanent Job Status Full-time Hours Per Week 37,5 Offer Starting Date 1 Mar 2026 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Reference Number SI4/PJI/2024-00172 Is the Job related to staff position within a Research Infrastructure? No Offer Description The Research Technician will be responsible for supporting, carrying out, and coordinating the experimental activities of the project, ensuring compliance with its objectives. Animal handling and experimentation: Management and maintenance of murine colonies. Performance of experimental procedures in mice subjected to high-fat diets and sepsis models. Metabolic characterization of colonies. Flow cytometry and spectral cytometry: Preparation of primary samples and/or cell lines and animal tissues for spectral cytometry analysis. Data analysis using different omic software (SpectroFlow, Omiq®, and FlowJo). Cell culture: Maintenance and experimentation with primary cell cultures, especially myeloid cells. Performance of functional assays (cell polarization, cytokine production, immune response assays, etc.). Molecular biology techniques: Mouse genotyping, nucleic acid extraction, and analysis by PCR and qPCR. Recording, analysis, and interpretation of molecular data. Microscopy and image analysis: Sample preparation for confocal microscopy. Image acquisition and quantitative analysis using ImageJ and/or Imaris. Continuous training and multidisciplinary collaboration: Participation in courses, seminars, and national/international conferences in the biomedical field. Coordinated work with predoctoral students and other technicians. Postgraduate Master’s degree in the field of biomedicine. Attendance at national and/or international conferences in the field of biomedicine (participation in talks, oral communications, and/or posters will be highly valued). Courses and/or micro-credentials (special consideration will be given to training in bioinformatics competencies, omics analysis, flow and spectral cytometry, statistical analysis using R, GraphPad, Python). Specific Requirements Special consideration will be given to a Master’s in Biomolecules and Cellular Dynamics. Certification for handling laboratory animals (Categories B and C). Languages ENGLISH Level Excellent Research Field Biological sciences Additional Information Eligibility criteria Previous work experience with murine models of high-fat diets and sepsis (endotoxemia), and metabolic characterization (GTT, ITT, PTT). Management of colonies with transgenic modifications. Experience with spectral flow cytometry techniques (Cytek Aurora 4L and 5L; SpectroFlow, Omiq®, FlowJo software) and handling of primary cell cultures (mainly myeloid cells) and endothelial cell lines. Experience in myeloid cell depletion techniques in murine models and molecular biology techniques (PCR, qPCR, genotyping, CRISPR). Experience in functional analyses of myeloid cells (polarization, cytokine analysis, etc.) and experience in confocal microscopy techniques and image analysis (ImageJ, Imaris software). Selection process Applications to this offer must compulsory be registered through the online application system of UAM. Nevertheless, prospective applicants are encouraged to contact the PI beforehand to discuss their suitability for the position, and to seek for assistance with the online application system. #J-18808-Ljbffr
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