Laboratory Fp or Higher Position

hace 3 semanas

Barcelona, España Vall d'Hebron Institut de Recerca A tiempo completo

VHIR-RRHH-FOR-003_vs01_

The Vall d'Hebron Research Institute
(VHIR) is a public sector institution that
promotes and develops the research,
innovation and biosanitary teaching of the
Vall d’Hebron University Hospital. Through
the excellence of our research, we identify
problems of society and we contribute to
spread them around the world.

In April 2015, the **Vall d’Hebron**
**Research Institute (VHIR) **obtained
the recognition of the European
Commission in **HR Excellence.**

This recognition proves that VHIR
endorses the general principles of the
European Charter for Researchers and
a Code of Conduct for the Recruitment
of Researchers **(Charter & Code)**.

**VHIR embraces Equality and**
**Diversity. As reflected in our values**
**we work toward ensuring inclusion**
**and equal opportunity in**
**recruitment, hiring, training, and**
**management for all staff within the**
**organization, regardless of gender,**
**civil status, family status, sexual**
**orientation, nationality, religion,**
**age, disability or race.
**Laboratory FP or Higher Position**
**Clinical Neuroimmunology Group
**The Clinical Neuroimmunology Group is part of the Centre d’Esclerosi Múltiple de Catalunya (Cemcat), a reference center
for multiple sclerosis (MS) at national and international levels. The Cemcat has a multidisciplinary team with more than
70 highly experienced professionals fully dedicated to clinical care, physical and cognitive rehabilitation, teaching, and

The Clinical Neuroimmunology Group searches for a laboratory technician to work on a project entitled “New human
induced pluripotent stem cell-based in vitro and in vivo models to study astrocyte pathology and neuroprotective strategies
in multiple sclerosis patients”, that is currently funded by the Instituto de Salud Carlos III.

Summary of the project: Multiple sclerosis (MS) has traditionally been considered an autoimmune inflammatory
neurological disorder in which the primary event is an aberrant immune response against central nervous system (CNS)
autoantigens in predisposed individuals. This leads to infiltration of peripheral blood cells into the CNS and results in
inflammatory demyelination and eventually axonal damage. While this probably holds true, in the present proposal we
will test the hypothesis that MS patients may have a primary defect in astrocytes that renders these cells dysfunctional
and more reactive to the autoimmune attack, making white matter lesions less prone to remyelinate. To this aim, we will
generate human induced pluripotent stem cells from patients with primary progressive MS and relapsing-remitting MS in
order to investigate human astrocyte pathology in an inflammatory environment in vitro and in vivo, through the
development of two new models created by: (i) exposing astrocytes in vitro to cerebrospinal fluid obtained from MS
patients with high inflammatory radiological phenotypes; (ii) transplanting human glial progenitor cells into the developing
mouse CNS and inducing experimental autoimmune encephalomyelitis. The proteomic signature of astroglial secretome
will be examined in vitro, under basal and inflammatory conditions. The genomic signature of MS human astrocytes during
EAE progression will be examined in vivo by spatial transcriptomics, to identify differentially expressed genes whose
expression may be modulated by small-molecule compounds with the potential to become future neuroprotective
therapies for patients with MS.
- VHIR-RRHH-FOR-003_vs01_

The Vall d'Hebron Research Institute
(VHIR) is a public sector institution that
promotes and develops the research,
innovation and biosanitary teaching of the
Vall d’Hebron University Hospital. Through
the excellence of our research, we identify
problems of society and we contribute to
spread them around the world.

In April 2015, the **Vall d’Hebron**
**Research Institute (VHIR) **obtained
the recognition of the European
Commission in **HR Excellence.**

This recognition proves that VHIR
endorses the general principles of the
European Charter for Researchers and
a Code of Conduct for the Recruitment
of Researchers **(Charter & Code)**.

**VHIR embraces Equality and**
**Diversity. As reflected in our values**
**we work toward ensuring inclusion**
**and equal opportunity in**
**recruitment, hiring, training, and**
**management for all staff within the**
**organization, regardless of gender,**
**civil status, family status, sexual**
**orientation, nationality, religion,**
**age, disability or race.**

**JOB DESCRIPTION**
**Education and qualifications**:
**Required:
Laboratory FP or higher.
Experience in maintaining cell cultures: passaging and freezing/thawing procedures.

**Experience and knowledge**:
**Desired**:
Experience in culturing induced pluripotent stem cells (maintenance, expansion and differentiation).

**Main responsibilities and duties**:
Main responsibilities and duties:
Maintaining and differentiation induced pluripotent stem cells from patient

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