Phd Student

hace 1 semana


España Universidad Católica A tiempo completo

Dentro del área de Investigación, se está llevando a cabo un proyecto de Investigación centrado en la Biología Molecular y Celular, dedicado a la ¨Investigación del efecto reafirmante del ácido oleanólico complejado con ciclodextrinas¨.

La persona seleccionada se encargaría de realizar las siguientes funciones:

- Conjugación del AO en ciclodextrinas tipo beta modificadas con grupos hidroxipropilo (CDs-HP-ß) y caracterización de los complejos formados AO/CD-HP-ß
- Producción, crecimiento y mantenimiento de líneas celulares y células primarias: células epiteliales Mv1Lu y fibroblastos primarios B118.
- Testado funcional de los complejos formados AO/CD-HP-ß sobre la línea celular Mv1Lu, realizando ensayos de heridas (wound healing scratch assay) para ver su efecto sobre la migración.
- Ensayos funcionales de heridas (wound healing scratch assay) en fibroblastos primarios humanos B118 testando los complejos AO/CD-HP-ß para medir su actividad biológica sobre la migración celular.
- Ensayos in vitro con los complejos AO/CD-HP-ß sobre los fibroblastos, estudio de proteínas marcadoras implicadas en migración celular mediante inmunotinción.

Within the Research area, a Research project focused on Molecular and Cell Biology is being carried out, dedicated to the Investigation of the firming effect of oleanolic acid complex with cyclodextrins.

The person selected would perform the following functions:

- Conjugation of AO in modified beta-type cyclodextrins with hydroxypropyl groups (CDs-HP-ß) and characterization of AO/CD-HP-ß-formed complexes;
- Production, growth and maintenance of cell lines and primary cells: Mv1Lu epithelial cells and primary fibroblasts B118.
- Functional testing of AO/CD-HP-ß-formed complexes on the Mv1Lu cell line, performing wound healing assay (wound healing scratch assay) tests to see their effect on migration.
- Functional wound tests (wound healing scratch assay) on human primary fibroblasts B118 testing AO/CD-HP-ß complexes to measure their biological activity on cell migration.
- In vitro trials with AO/CD-HP-ß complexes on fibroblasts, study of marker proteins involved in cell migration by immunostaining.

**Requisitos**:
- Máster Bioquímica, Biología Molecular y Biomedicina.
- Grado Biología, Biotecnología o relacionados.
- Inglés B2.
- Inquietud investigadora.
- Master’s Degree in Biochemistry, Molecular Biology and Biomedicine.
- Degree Biology, Biotechnology or related.
- English B2.
- Research concern.

Se ofrece

- Contrato predoctoral.
- Jornada Completa
- Pre-doctoral contract.
- Full-time



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