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Postdoctoral Researcher position
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España Centre for Genomic Regulation A tiempo completoThe Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. The CRG is composed by an interdisciplinary, motivated and creative scientific team which is supported both by a flexible and efficient administration and by high-end and...
Postdoctoral Researcher position
hace 1 mes
The InstituteThe Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. The CRG is composed of an interdisciplinary, motivated, and creative scientific team which is supported both by a flexible and efficient administration and by high-end and innovative technologies.In April 2021, the Centre for Genomic Regulation (CRG) received the renewal of the 'HR Excellence in Research' Award from the European Commission. This is a recognition of the Institute's commitment to developing an HR Strategy for Researchers, designed to bring the practices and procedures in line with the principles of the European Charter for Researchers and the Code of Conduct for the Recruitment of Researchers (Charter and Code).The roleWe are seeking a postdoctoral researcher to work with a team on the mechanism by which cells secrete the right amount and quality of bulky cargoes like collagens, mucins, and chylomicrons. Based on our data, we have suggested the involvement of transient tunnels that propel these bulk molecules from the endoplasmic reticulum lumen to the next compartment of the secretory pathway thus bypassing the use of a vesicular intermediate. TANGO1 and its binding partners mediate this event. A number of new genes potentially involved in this biochemical reaction have been identified. This role will require a mix of in vitro and in vivo analyses. Optogenetically controlled function of TANGO1 and inhibitors are being used to address the mechanism both in healthy and fibrotic tissues.About the labThe lab has a group dedicated to addressing the mechanism by which bulky cargoes are exported from metazoan cells (Raote and Malhotra. Ann. Rev. Biochem. 2021). Structural and functional analyses of the key components such as TANGO1, identified in our lab, are the focus of the studies. There are new gene products that likely function in the same pathway, but the mechanism of their action remains unaddressed. We aim to visualize and characterize the route taken by the bulky cargoes for their export from the ER. We are addressing the issue of how good collagens are selected while the misfolded collagens and mucins are degraded, and work is in progress to express the entire collagen production and secretion system in yeast. By addressing these issues, we ultimately aim to understand how cells control the secretion of these molecules in the right quantity and of the right quality. This is essential for the organization of cells in a tissue and a means to address collagen and mucin hypersecretion linked to human pathologies of tissue fibrosis.Whom would we like to hire?Professional experienceMust HaveMust have a comprehensive understanding and proven ability to modern approaches of molecular cell biology, imaging, genetic manipulation.All approaches of modern cell biology that include genetic manipulation of respective genes in metazoans, super-resolution microscopy, and publications to demonstrate expertise in the procedures mentioned.Desirable but not required/ Nice to haveYou have expertise in imaging.You have expertise in protein purification.You have experience and/or interest in working with animal models.You have experience with advanced bioinformatic tools (e.g. Python/R, omics data analysis).Education and trainingA Ph.D. in a relevant field of study and/or candidates with no more than one year of postdoctoral experience are also encouraged to apply. Demonstrated expertise through peer-reviewed publications in reputable journals is essential.LanguagesYou are fluent in English (written and spoken).Technical skillsModern approaches in molecular cell biology and super-resolution microscopy.CompetencesA demonstrated ability to organize and contribute original ideas to scientific research.Excellent scientific communication skills (demonstrated abilities in manuscript and grant writing, conference presentations, etc.).Strong organizational skills.The Offer – Working ConditionsContract duration: estimated until 31/8/2027.Estimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales.Target start date: As soon as possible.We provide a highly stimulating environment with state-of-the-art infrastructures, and unique professional career development opportunities. To check out our training and development portfolio, please visit our website in the training section.We offer and promote a diverse and inclusive environment and welcome applicants regardless of age, disability, gender, nationality, ethnicity, religion, sexual orientation, or gender identity.The CRG is committed to reconciling a work and family life of its employees and is offering an extended vacation period and the possibility to benefit from flexible working hours.
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