Experimental Postdoc
hace 2 meses
The Institute
The Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. The CRG is composed by an interdisciplinary, motivated and creative scientific team which is supported both by a flexible and efficient administration and by high-end and innovative technologies.
Please, check out our Recruitment Policy:
The role
About the team/ lab/ department
Tumors originate from normal cells that acquire tumor-initiating genetic events, such as translocations and somatic mutations. These genetic hits turn normal cells into pre-malignant cells, but do not lead to immediate tumor formation. For that, secondary genetic events as well as epigenetic hits are required.
We aim to create a better understanding of how epigenetic tumor-associated changes arise in the context of non-Hodgkin lymphomas. To that end, we aim to study the occurrence of these changes in healthy individuals as well as in pre-malignant cells in vitro (to be created by CRISPR/Cas9 genomic editing) and in vivo using single-cell technologies. Moreover, we aim to define cell intrinsic mechanisms, such as enhancer activation and 3D chromatin interactions that influence the occurrence of the observed changes.
Whom would we like to hire?
Professional experience
Must Have
Minimum of 3 years of hands-on experience in biomedical/biology related experimental work, including expertise in the following:
Genome-editing strategies in mammalian cells/cell lines (preferably in hematopoietic cells)
Complex cell transfection protocols and cloning
Epigenetic analyses (e.g. ChIP-seq, ATAC-seq, chromatin conformation capture)
Desirable but not required/ Nice to have
Hands-on expertise in:
Single-cell screenings
Optimisation of single-cell protocols to combine multiple read outs
Generation of next-generation sequencing libraries in different experimental contexts
Microscopy, preferable live-cell imaging, both at the level of the wetlab and analysis
Education and training
You hold a PhD in life sciences (biology, biotechnology, biomedicine, biophysics or a related field)
Languages
Fluency in English
Technical skills
Hands-on experience in the following areas:
CRISPR/Cas9-based technologies
Library preparation
Mammalian cell culture, including transfection methods for cell line based and/or primary samples and cloning
Epigenetic analyses
Optimisation of complex protocols
NGS methods
Competences
Highly developed organization skills
Excellent analytical and problem-solving skills
Creativity allowing to design experiments and explore data from unexplored angles
Enthusiasm, motivation and dedication to explore new scientific avenues
Capability to work on different projects simultaneously as part of a team and/or independently
The Offer - Working Conditions
Contract duration: 3 years
Estimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales
Target start date: Spring - Autumn 2024
We provide a highly stimulating environment with state-of-the-art infrastructures, and unique professional career development opportunities. To check out our training and development portfolio, please visit our website in the training section.
The CRG is committed to reconcile a work and family life of its employees and are offering extended vacation period and the possibility to benefit from flexible working hours.
Application Procedure
A motivation letter addressed to Dr R. Beekman
A complete CV including contact details.
Contact details of two referees.
Selection Process
"This project has received funding from the ERCEA program ERC-2021-STG under grant agreement No 101039265"
**Deadline**: February 25, 2024
**Job category**: Postdoc
**Area**: Life Sciences
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