(Aplica Ya) Lab Technician Position

hace 4 semanas


Barcelona, España Centre For Genomic Regulation A tiempo completo

.The Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. The CRG is composed of an interdisciplinary, motivated, and creative scientific team which is supported both by a flexible and efficient administration and by high-end and innovative technologies.We are seeking a highly motivated candidate to join our team to work on an interdisciplinary project. The candidate must master several techniques, such as general DNA and RNA manipulation techniques, purification, cloning, mutagenesis, NGS library preparation, PCR, RT-PCR, qPCR, chromatin immunoprecipitation, proteomic techniques (protein extraction, subcellular fractionation, western blot, protein-protein interaction techniques), FACS, and cell culture of primary and established cell lines.About the labUnderstanding the genetic basis of cancers has been a topic of intense research, and hundreds of gene mutations have been identified that can cause carcinogenesis. However, in the past few years, increasing evidence has suggested that mutations are not the only genetic changes that lead to cancer. Indeed, perturbations of chromatin structure and of other epigenetic mechanisms can cause inappropriate gene expression and genomic instability, resulting in cellular transformation and malignant outgrowth.Our research investigation is focused on understanding the role of several protein complexes that are involved in chromatin dynamics and metabolism, which when altered could participate in the establishment and maintenance of the aberrant silencing of tumor suppressor genes during transformation.Whom would we like to hire?Professional experienceMust HavePrevious experience in the field of molecular and cellular biology is required.A highly motivated and organized candidate.Capable of analyzing single-cell RNA seq data.Desirable but not required/Nice to have- You hold a PhD in Biology or equivalent.Education and training- You hold a Bachelor/Master's in Biology.Languages- You are proficient in English.Technical skillsMolecular Biology (general DNA and RNA manipulation techniques including purification, cloning, mutagenesis, PCR, RT-PCR, qPCR).Proteomic techniques (protein extraction, subcellular fractionation, western blot, protein-protein interaction techniques).Cell culture of primary and established cell lines.Advanced knowledge of bioinformatic analysis.CompetencesHighly developed organizational skills.You are capable of working in a group, and with a high degree of work autonomy.You are highly motivated.The Offer – Working ConditionsContract duration: Technical and scientific activities contract linked to the project (estimated duration 3.5 months).Estimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales.Target start date: as soon as possible



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