Predoctoral Research Assistant for the Kinases, Protein Phosphorylation and Cancer Group

hace 1 semana


Madrid, España CNIO A tiempo completo
Descripción

The group led by Iván Plaza-Menacho is looking for a PhD student position focused on the structure, function and pharmacology of protein kinases implicated in cancer. We are looking for a highly motivated candidate with a background in cell biology and tissue culture, biochemistry and structural biology.

Criterios de evaluación
  1. Education: Biochemistry, Biology or related areas.
  2. Experience: Proven experience in molecular biology, protein expression-purification, protein biochemistry as well as exposure to structural biology.
  3. Complementary skills: Technical knowledge and experience in other complementary disciplines such as cellular biology and signaling will be very positively valued.
  4. Lab skills: Good lab technical skills and abilities, versatile and multifaceted researcher able to multitask.
  5. Technical skills:
    1. Molecular biology (cloning and mutagenesis).
    2. Tissue culture (insect cells and cancer cell lines).
    3. Protein expression in different heterologous systems (bacterial and/or insect cells using baculovirus) and purification affinity, size-exclusion and ionic-exchange chromatography.
    4. Protein biochemistry (WBs and phosphorylation assays) and biophysics (enzymatic assays, DSF, calorimetry, AUC).
  6. Language skills: Good level of English speaking and writing.
  7. Documentation: Detailed documentation of all experimental work is essential.
  8. Interpersonal skills: Good communication and teamwork skills.
Se ofrece
  1. The opportunity to be part of a Cancer Research Centre of excellence.
  2. Competitive salary.
  3. An energetic working environment.
  4. Contract linked to a project.
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