Experimental Postdoc
hace 2 semanas
The Institute
The Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries.
The CRG is composed by an interdisciplinary, motivated and creative scientific team which is supported both by a flexible and efficient administration and by high-end and innovative technologies.
In April 2021, the Centre for Genomic Regulation (CRG) received the renewal of the 'HR Excellence in Research' Award from the European Commission.
This is a recognition of the Institute's commitment to developing an HR Strategy for Researchers, designed to bring the practices and procedures in line with the principles of the European Charter for Researchers and the Code of Conduct for the Recruitment of Researchers (Charter and Code).
Please, check out our Recruitment PolicyThe role
About the team/ lab/ department**Tumors originate from normal cells that acquire tumor-initiating genetic events, such as translocations and somatic mutations. These genetic hits turn normal cells into pre-malignant cells, but do not lead to immediate tumor formation. For that, secondary genetic events as well as epigenetic hits are required.
We aim to create a better understanding of how epigenetic tumor-associated changes arise in the context of non-Hodgkin lymphomas.
To that end, we aim to study the occurrence of these changes in healthy individuals as well as in pre-malignant cells in vitro (to be created by CRISPR/Cas9 genomic editing) and in vivo using single-cell technologies.
Moreover, we aim to define cell intrinsic mechanisms, such as enhancer activation and 3D chromatin interactions that influence the occurrence of the observed changes.
Whom would we like to hire?**
Professional experience
Must Have
- Minimum of 3 years of handson experience in biomedical/biology related experimental work, including expertise in the following:
- Genome-editing strategies in mammalian cells/cell lines (preferably in hematopoietic cells)
- Complex cell transfection protocols and cloning
- Epigenetic analyses (e.g. ChIPseq, ATACseq, chromatin conformation capture)
Desirable but not required/ Nice to have**Hands-on expertise in:
- Single
- cell screenings
- Optimisation of singlecell protocols to combine multiple read outs
- Generation of nextgeneration sequencing libraries in different experimental contexts
- Microscopy, preferable livecell imaging, both at the level of the wetlab and analysis
Education and training
- You hold a PhD in life sciences (biology, biotechnology, biomedicine, biophysics or a related field)
Languages
- Fluency in English
Technical skills
Hands-on experience in the following areas:
- CRISPR/Cas9-based technologies
- Library preparation
- Mammalian cell culture, including transfection methods for cell line based and/or primary samples and cloning
- Epigenetic analyses
- Optimisation of complex protocols
- NGS methods
Competences
- Highly developed organization skills
- Excellent analytical and problemsolving skills
- Creativity allowing to design experiments and explore data from unexplored angles
- Enthusiasm, motivation and dedication to explore new scientific avenues
- Capability to work on different projects simultaneously as part of a team and/or independently
The Offer - Working Conditions**-
Contract duration**: 3 years
-
Estimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales
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Target start date**: Spring
- Autumn 2024
We offer and promote a diverse and inclusive environment** and welcomes applicants regardless of age, disability, gender, nationality, ethnicity, religion, sexual orientation or gender identity.
The CRG is committed to reconcile a work and family life** of its employees and are offering extended vacation period and the possibility to benefit from flexible working hours.
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