Laboratory Technician in Inflammation, Tissue
hace 2 semanas
Created in 2005 by the Generalitat de Catalunya (Government of Catalonia) and the University of Barcelona, IRB Barcelona is a Severo Ochoa Centre of Excellence—a seal that was awarded in 2011.
The institute is devoted to conducting research of excellence in biomedicine and to transferring results to clinical practice, thus improving people's quality of life, while simultaneously promoting the training of outstanding researchers, technology transfer, and public communication of science.
Its 28 laboratories and seven core facilities address basic questions in biology and are orientated to diseases such as cancer, metastasis, Alzheimer's, diabetes, and rare conditions.
IRB Barcelona is an international centre that hosts 400 members and 35 nationalities. It is located in the Barcelona Science Park.IRB Barcelona forms part of the Barcelona Institute of Science and Technology (BIST) and the "Xarxa de Centres de Recerca de Catalunya" (CERCA).
**_IRB Barcelona is seeking a motivated, conscientious, and enthusiastic scientist to join the Inflammation, Tissue Plasticity & Cancer laboratory (Dr. Direna Alonso-Curbelo) as a Laboratory Technician.
_**- Our lab aims to understand how oncogenic mutations and environmental insults alter normal cellular identity and tissue homeostasis to drive and sustain cancer, with a focus on pancreatic and liver malignancies.
To do so, we combine functional genomics tools (CRISPR/Cas9; RNAi), mouse models and organoid/cell culture systems, and (epi)genomic methods including single-cell technologies.
__Starting date:
early october 2023 or as soon as possible._
- This position will be partially covered by this position will be partially covered by the project PID OA-I00: "Dissecting and targeting early epigenetic programs in pancreatic cancer (PRE-CAN)"._
DUTIES:
- Implement established and new experimental approaches and perform organizational duties under mínimal supervision.
Research activities may include (but are not limited to) DNA/RNA extraction, vector cloning, mouse handling, minor surgeries (pancreas, liver), iv/ip injections, tissue processing, cell line/organoid culture, genetic manipulation (CRISPR/Cas9, RNAi), genotyping, preparation of lab reagents, qRT
- PCR, immunoblotting, flow cytometry and histology (IF/IHC), assisting with the establishment and implementation of experimental pipelines to support lab goals and their related SOPs, performing basic statistical data analysis and literature search to contribute with new ideas on topics related to the lab's research mission.
Operational activities may include (but are not limited to) monitoring and ordering supplies; operating and trouble-shooting lab equipment; organizing data repositories, lab freezers/fridges, and chemical inventory; managing mouse colony; assisting team members in planning or execution of experiments; and contributing to an enriching, positive and inclusive lab environment.
-
Team dynamics: While prior expertise in the above activities will be positively valued, there will be ample opportunities for learning/training in the conceptual and experimental fronts driving our research forward. Thus, we are looking for a proactive scientist who will contribute to the lab's goals with their expertise and is also committed to learning new technologies and concepts. The technician will also have opportunities to oversee and train undergraduate students and work with the PI to mentor junior lab members.
EXPERIENCE, KNOWLEDGE, SKILLS:
Must Have - Required:
-
Experience:
- Bachelor's or Master's degree in molecular/cell biology, immunology, chemistry, biomedicine or other scientific disciplines in a related field; or FP degree in a related field and two (2) years of related experience.
- Animal experimentation training, with handson expertise in rodenthandling procedures. Practical experience with survival surgeries and/or colony management is a plus.
Knowledge:
- Laboratory techniques, equipment, terminology, and support processes and practices.
- Basic molecular techniques.
- Data organization, statistical analysis, and computer literacy.
- Prior experience in CRISPR/Cas9based technologies, epigenomic (RNA/ATAC/ChIPseq), proteomics, and/or singlecell analyses (data generation and/or computational analyses) will be positively valued.
Skills:
- Interpersonal and effective communication skills.
- Motivated to learn new techniques.
- Ability to work independently well as a team member.
- Highly organized and proactive attitude.
- Ability to perform multiple tasks independently.
- Time
-
Languages:Proficient in English (fluent, oral and written).
Desirable:
-
Experience:
- Prior experience in competitive research environments.
- Background in cancer, immunology, neurobiology and/or epigenetics.
Technical Skils:
- Epigenomic analyses and NGS methods (eg: ATACseq, ChI
-
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